Rifamycins do not function by allosteric modulation of binding of Mg 2+ to the RNA polymerase active center Academic Article uri icon

Overview

MeSH Major

  • Anti-Bacterial Agents
  • DNA-Directed RNA Polymerases
  • Magnesium
  • Rifamycins

abstract

  • Rifamycin antibacterial agents inhibit bacterial RNA polymerase (RNAP) by binding to a site adjacent to the RNAP active center and preventing synthesis of RNA products >2-3 nt in length. Recently, Artsimovitch et al. [(2005) Cell 122:351-363] proposed that rifamycins function by allosteric modulation of binding of Mg(2+) to the RNAP active center and presented three lines of biochemical evidence consistent with this proposal. Here, we show that rifamycins do not affect the affinity of binding of Mg(2+) to the RNAP active center, and we reassess the three lines of biochemical evidence, obtaining results not supportive of the proposal. We conclude that rifamycins do not function by allosteric modulation of binding of Mg(2+) to the RNAP active center.

publication date

  • September 30, 2008

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2567451

Digital Object Identifier (DOI)

  • 10.1073/pnas.0802822105

PubMed ID

  • 18787125

Additional Document Info

start page

  • 14820

end page

  • 5

volume

  • 105

number

  • 39