Chapter Eighteen Methods to Dissect Mitochondrial Membrane Permeabilization in the Course of Apoptosis Report uri icon

Overview

MeSH Major

  • Carrier Proteins
  • Immunity, Innate
  • Inflammasomes
  • Mitochondria

abstract

  • In several paradigms of cell death, mitochondrial membrane permeabilization (MMP) delimits the frontier between life and death. Mitochondria control the intrinsic pathway of apoptosis and participate in the extrinsic pathway. Moreover, they have been implicated in nonapoptotic cell death modalities. Irrespective of its initiation at the inner or the outer mitochondrial membrane (IM and OM, respectively), MMP culminates in the functional (dissipation of the mitochondrial transmembrane potential, shutdown of ATP synthesis, redox imbalance) and structural (reorganization of cristae, release of toxic intermembrane space proteins into the cytosol) collapse of mitochondria. This has a profound impact on cellular metabolism, activates caspase-dependent and -independent executioner mechanisms, and finally results in the demise of the cell. However, the partial and/or temporary permeabilization of one or both mitochondrial membranes is not always a prelude to cell death. This chapter proposes a method and several guidelines to discriminate between IM and OM permeabilization and to identify MMP that does indeed precede cell death. This approach relies on the integration of currently available techniques and may be easily introduced in the laboratory routine for a more precise detection of cell death.

publication date

  • September 26, 2008

Research

keywords

  • Report

Identity

Digital Object Identifier (DOI)

  • 10.1016/S0076-6879(08)01418-3

Additional Document Info

start page

  • 355

end page

  • 74

volume

  • 442