IFN-γ enables cross-presentation of exogenous protein antigen in human Langerhans cells by potentiating maturation Academic Article uri icon

Overview

MeSH Major

  • Interferon-gamma
  • Langerhans Cells

abstract

  • We compared monocyte-derived dendritic cells and transforming growth factor-beta1-induced Langerhans-like cells (LCs) for their capacity to cross-present exogenous NY-ESO-1 protein/antibody immune complexes to an NY-ESO-1-specific CD8+ T cell clone. In contrast to dendritic cells, LCs were not able to cross-present NY-ESO-1 to the T cell clone constitutively but did so after treatment with IFN-gamma. Remarkably, this IFN-gamma-inducible characteristic was due neither to enhanced antigen uptake nor to facilitated antigen processing in LCs. Rather, IFN-gamma acted at least in part by potentiating the maturation of otherwise refractory LCs, enabling in turn exogenous antigen to reach the processing machinery. This model of conditional cross-presentation establishes an original level of action for IFN-gamma as an effective immune modulator and supports the use of IFN-gamma in protein vaccination strategies targeting LCs.

publication date

  • October 5, 2004

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC521945

Digital Object Identifier (DOI)

  • 10.1073/pnas.0405947101

PubMed ID

  • 15383663

Additional Document Info

start page

  • 14467

end page

  • 72

volume

  • 101

number

  • 40