O6-Methylguanine-DNA methyltransferase in human cells Academic Article Article uri icon

Overview

MeSH Major

  • Gastric Mucosa
  • Gastrointestinal Diseases
  • Intestinal Mucosa
  • Oxidative Stress
  • Reactive Oxygen Species

abstract

  • O6-Methylguanine-DNA methyltransferase activity was measured in extracts of human tumor cells and was partially purified from human placenta. Repair of O6-methylguanine in DNA inactivated the methyltransferase, and treatment of cells with MNNG, which produces this alkylated base in DNA, depleted the cells of active methyltransferase. RNA and protein synthesis were required for restoration of methyltransferase activity, which transiently exceeded the original levels by 50% 48 h after treatment. One species of methyltransferase of Mr = 22 kd was present in human tumor cells and human placenta. © 1984.

publication date

  • January 1984

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0167-8817(84)90044-0

PubMed ID

  • 6694657

Additional Document Info

start page

  • 27

end page

  • 36

volume

  • 131

number

  • 1