Does Rft1 flip an N-glycan lipid precursor? Comment uri icon

Overview

MeSH Major

  • Dolichol
  • Mannans
  • Membrane Glycoproteins
  • Phospholipid Transfer Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins

abstract

  • Protein N-glycosylation requires flipping of the glycolipid Man(5)GlcNAc(2)-diphosphate dolichol (Man(5)GlcNAc(2)-PP-Dol) across the endoplasmic reticulum (ER). Helenius et al. report genetic evidence suggesting that Rft1, an essential ER membrane protein in yeast, is required directly to translocate Man(5)GlcNAc(2)-PP-Dol. We now show that a specific ER protein(s), but not Rft1, is required to flip Man(5)GlcNAc(2)-PP-Dol in reconstituted vesicles. Rft1 may have a critical accessory role in translocating Man(5)GlcNAc(2)-PP-Dol in vivo, but the Man(5)GlcNAc(2)-PP-Dol flippase itself remains to be identified.

publication date

  • July 31, 2008

Research

keywords

  • Comment

Identity

Digital Object Identifier (DOI)

  • 10.1038/nature07165

PubMed ID

  • 18668045

Additional Document Info

start page

  • E3

end page

  • 4; discussion E4-5

volume

  • 454

number

  • 7204