Detection of zinc ions by differential circularly polarized fluorescence excitation Academic Article uri icon

Overview

MeSH Major

  • Fluorescence Polarization
  • Zinc

abstract

  • A new chiroptical spectroscopic approach, differential circularly polarized fluorescence excitation (CPE), can be used to provide a selective method for detecting the presence of zinc ions. The approach utilizes the same instrumentation as fluorescence-detected circular dichroism and provides strong contrast in metal detection due to response to both chelation-enhanced fluorescence and circular dichroism upon metal ion binding. The observed contrast is therefore better than either of the parent spectroscopic detection methods. CPE also provides a strategy to reduce interference from background such as protein-based tryptophan fluorescence.

publication date

  • September 29, 2004

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1021/ja047213z

PubMed ID

  • 15382888

Additional Document Info

start page

  • 11760

end page

  • 1

volume

  • 126

number

  • 38