Overexpression of Syk tyrosine kinase in peripheral T-cell lymphomas Academic Article uri icon

Overview

MeSH Major

  • Intracellular Signaling Peptides and Proteins
  • Lymphoma, T-Cell, Peripheral
  • Protein-Tyrosine Kinases

abstract

  • Peripheral T-cell lymphomas (PTCLs) are fatal in the majority of patients and novel treatments, such as protein tyrosine kinase (PTK) inhibition, are needed. The recent finding of SYK/ITK translocations in rare PTCLs led us to examine the expression of Syk PTK in 141 PTCLs. Syk was positive by immunohistochemistry (IHC) in 133 PTCLs (94%), whereas normal T cells were negative. Western blot on frozen tissue (n=6) and flow cytometry on cell suspensions (n=4) correlated with IHC results in paraffin. Additionally, western blot demonstrated that Syk-positive PTCLs show tyrosine (525/526) phosphorylation, known to be required for Syk activation. Fluorescence in situ hybridization showed no SYK/ITK translocation in 86 cases. Overexpression of Syk, phosphorylation of its Y525/526 residues and the availability of orally available Syk inhibitors suggest that Syk merits further evaluation as a candidate target for pharmacologic PTK inhibition in patients with PTCL.

publication date

  • June 2008

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2778211

Digital Object Identifier (DOI)

  • 10.1038/leu.2008.77

PubMed ID

  • 18401419

Additional Document Info

start page

  • 1139

end page

  • 43

volume

  • 22

number

  • 6