Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line - NCL-SG3 Academic Article uri icon


MeSH Major

  • Chlorides
  • Epithelial Cells
  • Gene Expression
  • Receptor, PAR-2


  • Proteinase-activated receptor 2 (PAR-2) has been shown to elicit secretion in a variety of secretory epithelial cells by the transepithelial movement of chloride ions across the apical membrane. However, it is not known whether these receptors are present and/or functional in the secretory epithelial cells of the human eccrine sweat gland. To investigate this possibility mRNA analysis, Ca2+ microspectrofluorimetry and the short circuit current (Isc) technique were used to quantify electrolyte transport in a cell line (NCL-SG3) derived from human eccrine sweat gland secretory epithelia. The results provided molecular and functional evidence of the presence of PAR-2 receptors in the NCL-SG3 cells and show that these receptors can activate transepithelial Cl- secretion possibly via Ca2+-activated Cl- channels.

publication date

  • June 2008



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0625.2007.00659.x

PubMed ID

  • 18070079

Additional Document Info

start page

  • 505

end page

  • 11


  • 17


  • 6