Genomic and Proteomic Analysis of phiEco32, a Novel Escherichia coli Bacteriophage Academic Article uri icon

Overview

MeSH Major

  • Coliphages
  • Genome, Viral
  • Proteome

abstract

  • A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.

publication date

  • March 28, 2008

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2587145

Digital Object Identifier (DOI)

  • 10.1016/j.jmb.2007.12.077

PubMed ID

  • 18294652

Additional Document Info

start page

  • 774

end page

  • 89

volume

  • 377

number

  • 3