The inhibition of tumor cell intravasation and subsequent metastasis via regulation of in vivo tumor cell motility by the tetraspanin CD151. Academic Article uri icon

Overview

MeSH

  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD151
  • Binding Sites, Antibody
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Proliferation
  • Chick Embryo
  • Extracellular Matrix
  • Humans
  • Mice
  • Mice, Knockout
  • Mice, SCID
  • Microscopy, Fluorescence
  • Microscopy, Video
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Polymerase Chain Reaction
  • RNA Interference
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Time Factors
  • Transfection

MeSH Major

  • Antigens, CD
  • Cell Movement
  • Chorioallantoic Membrane
  • Neoplasms, Experimental

abstract

  • In vivo tumor cell migration through integrin-dependent pathways is key to the metastatic behavior of malignant cells. Using quantitative in vivo assays and intravital imaging, we assessed the impact of cell migration, regulated by the integrin-associated tetraspanin CD151, on spontaneous human tumor cell metastasis. We demonstrate that promoting immobility through a CD151-specific metastasis blocking mAb prevents tumor cell dissemination by inhibiting intravasation without affecting primary tumor growth, tumor cell arrest, extravasation, or growth at the secondary site. In vivo, this loss of migration is the result of enhanced tumor cell-matrix interactions, promoted by CD151, which prevent dissociation by individual cells and leads to a subsequent inhibition of invasion and intravasation at the site of the primary tumor.

publication date

  • March 2008

has subject area

  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, CD151
  • Binding Sites, Antibody
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Chick Embryo
  • Chorioallantoic Membrane
  • Extracellular Matrix
  • Humans
  • Mice
  • Mice, Knockout
  • Mice, SCID
  • Microscopy, Fluorescence
  • Microscopy, Video
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Neoplasms, Experimental
  • Polymerase Chain Reaction
  • RNA Interference
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Time Factors
  • Transfection

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3068919

Digital Object Identifier (DOI)

  • 10.1016/j.ccr.2008.01.031

PubMed ID

  • 18328426

Additional Document Info

start page

  • 221

end page

  • 234

volume

  • 13

number

  • 3