Optical microscopy-based migration assay for human neutrophils. Academic Article uri icon

Overview

MeSH Major

  • Cell Migration Assays
  • Microscopy, Interference
  • Neutrophils

abstract

  • This unit describes an in vitro microscopy assay for examining the migration of human neutrophils in two dimensions to identify the underlying cause of a migration defect and to evaluate a variety of migration parameters that cannot be studied using migration through a porous filter. Freshly isolated human neutrophils a placed in the chamber, stimulated, and images are collected at various time points. The data can be used to determine the effects of a pharmacological treatment on the migration of individual cells.

publication date

  • February 2003

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 18228419

Additional Document Info

start page

  • Unit 12.6

volume

  • Chapter 12