Affinity isolation and I-DIRT mass spectrometric analysis of the Escherichia coli O157:H7 sakai RNA polymerase complex Academic Article uri icon

Overview

MeSH Major

  • Chromatography, Affinity
  • DNA-Directed RNA Polymerases
  • Escherichia coli O157
  • Escherichia coli Proteins
  • Mass Spectrometry

abstract

  • Bacteria contain a single multisubunit RNA polymerase that is responsible for the synthesis of all RNA. Previous studies of the Escherichia coli K-12 laboratory strain identified a group of effector proteins that interact directly with RNA polymerase to modulate the efficiency of transcription initiation, elongation, or termination. Here we used a rapid affinity isolation technique to isolate RNA polymerase from the pathogenic Escherichia coli strain O157:H7 Sakai. We analyzed the RNA polymerase enzyme complex using mass spectrometry and identified associated proteins. Although E. coli O157:H7 Sakai contains more than 1,600 genes not present in the K-12 strain, many of which are predicted to be involved in transcription regulation, all of the identified proteins in this study were encoded on the "core" E. coli genome.

publication date

  • February 2008

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2238195

Digital Object Identifier (DOI)

  • 10.1128/JB.01599-07

PubMed ID

  • 18083804

Additional Document Info

start page

  • 1284

end page

  • 9

volume

  • 190

number

  • 4