Evolutionarily conserved gene family important for fat storage Academic Article uri icon

Overview

MeSH Major

  • Adipose Tissue
  • Evolution, Molecular
  • Gene Expression Regulation
  • Liver
  • Membrane Proteins

abstract

  • The ability to store fat in the form of cytoplasmic triglyceride droplets is conserved from Saccharomyces cerevisiae to humans. Although much is known regarding the composition and catabolism of lipid droplets, the molecular components necessary for the biogenesis of lipid droplets have remained obscure. Here we report the characterization of a conserved gene family important for lipid droplet formation named fat-inducing transcript (FIT). FIT1 and FIT2 are endoplasmic reticulum resident membrane proteins that induce lipid droplet accumulation in cell culture and when expressed in mouse liver. shRNA silencing of FIT2 in 3T3-LI adipocytes prevents accumulation of lipid droplets, and depletion of FIT2 in zebrafish blocks diet-induced accumulation of lipid droplets in the intestine and liver, highlighting an important role for FIT2 in lipid droplet formation in vivo. Together these studies identify and characterize a conserved gene family that is important in the fundamental process of storing fat.

publication date

  • January 8, 2008

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2224239

Digital Object Identifier (DOI)

  • 10.1073/pnas.0708579105

PubMed ID

  • 18160536

Additional Document Info

start page

  • 94

end page

  • 9

volume

  • 105

number

  • 1