An antibody-desferrioxamine conjugate labelled with 67Ga. Academic Article uri icon

Overview

abstract

  • The optimum conditions for producing a 67Ga labelled antibody-desferrioxamine conjugate were determined. The mouse monoclonal antibody LICR-LON-M8 was coupled to the metal chelating compound desferrioxamine (DFO) using glutaraldehyde (GLUT). A DFO:GLUT:M8 molar ratio of 500:150:1 gave an immunoreactive antibody with low amounts (less than 5%) of high molecular weight polymer. The labelling efficiency with 67 Ga was greater than 90%, with a specific activity of 2-4 MBq/mg antibody. This 67Ga labelled antibody is suitable for evaluation as a diagnostic imaging agent.

publication date

  • January 1, 1986

Research

keywords

  • Antibodies, Monoclonal
  • Deferoxamine
  • Gallium Radioisotopes

Identity

Digital Object Identifier (DOI)

  • 10.1016/0883-2897(86)90128-5

PubMed ID

  • 3818312

Additional Document Info

volume

  • 13

issue

  • 5