Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode Academic Article uri icon

Overview

MeSH Major

  • Endocytosis
  • Exocytosis
  • Microscopy, Fluorescence
  • Synaptic Vesicles

abstract

  • The nature of synaptic vesicle recycling at nerve terminals has been a subject of considerable debate for >35 years. Here, we report the use of an optical strategy that allows the exocytosis and retrieval of synaptic components to be tracked in real time at single-molecule sensitivity in living nerve terminals. This approach has allowed us to examine the recycling of synaptic vesicles in response to single action potentials. Our results show that, after exocytosis, individual synaptic vesicles are retrieved by a stochastic process with an exponential distribution of delay times, with a mean time of approximately 14 s. We propose that evidence for fast endocytosis, such as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic nature of a slower process.

publication date

  • December 18, 2007

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2154473

Digital Object Identifier (DOI)

  • 10.1073/pnas.0707574105

PubMed ID

  • 18077369

Additional Document Info

start page

  • 20576

end page

  • 81

volume

  • 104

number

  • 51