Distinctive gene expression signatures in rheumatoid arthritis synovial tissue fibroblast cells: correlates with disease activity. Academic Article uri icon

Overview

MeSH

  • Adult
  • Aged
  • Blood Sedimentation
  • C-Reactive Protein
  • Cells, Cultured
  • Disease Progression
  • Female
  • Humans
  • Joints
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Osteoarthritis
  • Rheumatoid Factor
  • Surveys and Questionnaires

MeSH Major

  • Arthritis, Rheumatoid
  • Fibroblasts
  • Gene Expression Profiling
  • Synovial Membrane

abstract

  • Gene expression profiling of rheumatoid arthritis (RA) and osteoarthritis (OA) joint tissue samples provides a unique insight into the gene signatures involved in disease development and progression. Fibroblast-like synovial (FLS) cells were obtained from RA, OA and control trauma joint tissues (non-RA, non-OA) and RNA was analyzed by Affymetrix microarray. Thirty-four genes specific to RA and OA FLS cells were identified (P<0.05). HOXD10, HOXD11, HOXD13, CCL8 and LIM homeobox 2 were highly and exclusively expressed in RA and CLU, sarcoglycan-gamma, GPR64, POU3F3, peroxisome proliferative activated receptor-gamma and tripartite motif-containing 2 were expressed only in OA. The data also revealed expression heterogeneity for patients with the same disease. To address disease heterogeneity in RA FLS cells, we examined the effects of clinical disease parameters (Health Assessment Questionnaire (HAQ) score, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF)) and drug therapies (methotrexate/prednisone) on RA FLS cell gene expression. Eight specific and unique correlations were identified: human leukocyte antigen (HLA)-DQA2 with HAQ score; Clec12A with RF; MAB21L2, SIAT7E, HAPLN1 and BAIAP2L1 with CRP level; RGMB and OSAP with ESR. Signature RA FLS cell gene expression profiles may provide insights into disease pathogenesis and have utility in diagnosis, prognosis and drug responsiveness.

publication date

  • September 2007

has subject area

  • Adult
  • Aged
  • Arthritis, Rheumatoid
  • Blood Sedimentation
  • C-Reactive Protein
  • Cells, Cultured
  • Disease Progression
  • Female
  • Fibroblasts
  • Gene Expression Profiling
  • Humans
  • Joints
  • Male
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis
  • Osteoarthritis
  • Rheumatoid Factor
  • Surveys and Questionnaires
  • Synovial Membrane

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/sj.gene.6364400

PubMed ID

  • 17568789

Additional Document Info

start page

  • 480

end page

  • 491

volume

  • 8

number

  • 6