Oligonucleotide microarray design for detection and serotyping of human respiratory adenoviruses by using a virtual amplicon retrieval software. Academic Article uri icon

Overview

MeSH

  • Adenovirus Infections, Human
  • Child
  • Humans
  • Respiratory Tract Infections
  • Sensitivity and Specificity

MeSH Major

  • Adenoviruses, Human
  • Nasopharynx
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Serotyping
  • Software

abstract

  • The rapid development of new molecular biology methods has improved infectious disease diagnosis, which is increasingly important to clinical management and public health. A wide variety of new methods which are more specific, sensitive and robust, such as combination of PCR and microarray technology, has gradually replaced the conventional assays usually used in routine diagnosis. Both methods have the advantage of speed and sensitivity but tend to be expensive and technically demanding. Therefore, it is necessary to develop more simple assays that could be available for all diagnostic laboratories. To this aim, a simple microarray assay for detection and typing of adenoviruses causing acute respiratory disease in humans was developed. The absence of effective therapeutic or alternative prophylactic treatment for this infection makes essential its rapid diagnosis to implement fast control measures. Moreover, the family Adenoviridae includes numerous serotype groups and constitutes an ideal model system to develop diagnostic methods for other human pathogens. For their precise identification, an amplicon retrieval software that simplifies and accelerates the processing of all sequences necessary to perform this type of experiments has also been developed. This new technology was applied for the simultaneous detection and serotyping of acute respiratory disease-causing adenoviruses in laboratory and clinical samples with efficiency and accuracy.

publication date

  • November 2007

has subject area

  • Adenovirus Infections, Human
  • Adenoviruses, Human
  • Child
  • Humans
  • Nasopharynx
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Respiratory Tract Infections
  • Sensitivity and Specificity
  • Serotyping
  • Software

Research

keywords

  • Journal Article
  • Validation Studies

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.jviromet.2007.05.018

PubMed ID

  • 17586060

Additional Document Info

start page

  • 127

end page

  • 136

volume

  • 145

number

  • 2