A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing Academic Article uri icon

Overview

MeSH Major

  • Base Sequence
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Library
  • MicroRNAs

abstract

  • MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

authors

publication date

  • June 29, 2007

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2681231

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2007.04.040

PubMed ID

  • 17604727

Additional Document Info

start page

  • 1401

end page

  • 14

volume

  • 129

number

  • 7