Responses of the human airway epithelium transcriptome to in vivo injury. Academic Article Article uri icon

Overview

MeSH

  • Adult
  • Female
  • Humans
  • Male
  • Microarray Analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors

MeSH Major

  • Gene Expression
  • Gene Expression Profiling
  • Respiratory Mucosa
  • Wound Healing

abstract

  • To identify genes participating in human airway epithelial repair, we used bronchoscopy and brushing to denude the airway epithelium of healthy individuals, sequentially sampled the same region 7 and 14 days later, and assessed gene expression by Affymetrix microarrays with TaqMan RT-PCR confirmation. Histologically, the injured area was completely covered by a partially redifferentiated epithelial layer after 7 days; by 14 days the airway epithelium was very similar to the uninjured state. At day 7 compared with resting epithelium, there were substantial differences in gene expression pattern, with a distinctive airway epithelial "repair transcriptome" of actively proliferating cells in the process of redifferentiation. The repair transcriptome at 7 days was dominated by cell cycle, signal transduction, metabolism and transport, and transcription genes. Interestingly, the majority of differentially expressed cell cycle genes belonged to the G2 and M phases, suggesting that the proliferating cells were relatively synchronized 1 wk following injury. At 14 days postinjury, the expression profile was similar to that of resting airway epithelium. These observations provide a baseline of the functional gene categories participating in the process of normal human airway epithelial repair that can be used in future studies of injury and repair in airway epithelial diseases.

publication date

  • April 24, 2007

has subject area

  • Adult
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Male
  • Microarray Analysis
  • Respiratory Mucosa
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Wound Healing

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1152/physiolgenomics.00167.2006

PubMed ID

  • 17164391

Additional Document Info

start page

  • 139

end page

  • 148

volume

  • 29

number

  • 2