FLIP inhibits endothelial cell apoptosis during hyperoxia by suppressing Bax. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cells, Cultured
  • Down-Regulation
  • Endothelial Cells
  • Mice
  • Phosphorylation
  • Protein Kinase C
  • Reactive Oxygen Species
  • p38 Mitogen-Activated Protein Kinases

MeSH Major

  • Apoptosis
  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • Hyperoxia
  • Lung
  • Respiratory Mucosa
  • bcl-2-Associated X Protein

abstract

  • High oxygen tension (hyperoxia) causes pulmonary cell death, involving apoptosis, necrosis, or mixed death phenotypes, though the underlying mechanisms remain unclear. In mouse lung endothelial cells (MLEC) hyperoxia activates both extrinsic (Fas-dependent) and intrinsic (mitochondria-dependent) apoptotic pathways. We examined the hypothesis that FLIP, an inhibitor of caspase-8, can protect endothelial cells against the lethal effects of hyperoxia. Hyperoxia caused the time-dependent downregulation of FLIP in MLEC. Overexpression of FLIP attenuated intracellular reactive oxygen species generation during hyperoxia exposure, by downregulating extracellular-regulated kinase-1/2 activation and p47(phox) expression. FLIP prevented hyperoxia-induced trafficking of the death-inducing signal complex from the Golgi apparatus to the plasma membrane. Furthermore, FLIP blocked the activations of caspase-8/Bid, caspases -3/-9, and inhibited the mitochondrial translocation and activation of Bax, resulting in protection against hyperoxia-induced cell death. Under normoxic conditions, FLIP expression increased the phosphorylation of p38 mitogen-activated protein kinase leading to increased phosphorylation of Bax during hyperoxic stress. Furthermore, FLIP expression markedly inhibited protein kinase C activation and expression of distinct protein kinase C isoforms (alpha, eta, and zeta), and stabilized an interaction of PKC with Bax. In conclusion, FLIP exerted novel inhibitory effects on extrinsic and intrinsic apoptotic pathways, which significantly protected endothelial cells from the lethal effects of hyperoxia.

publication date

  • May 15, 2007

has subject area

  • Animals
  • Apoptosis
  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • Cells, Cultured
  • Down-Regulation
  • Endothelial Cells
  • Hyperoxia
  • Lung
  • Mice
  • Phosphorylation
  • Protein Kinase C
  • Reactive Oxygen Species
  • Respiratory Mucosa
  • bcl-2-Associated X Protein
  • p38 Mitogen-Activated Protein Kinases

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.freeradbiomed.2007.02.020

PubMed ID

  • 17448907

Additional Document Info

start page

  • 1599

end page

  • 1609

volume

  • 42

number

  • 10