Differential ability of Ptf1a and Ptf1a-VP16 to convert stomach, duodenum and liver to pancreas. Academic Article uri icon

Overview

MeSH

  • Animals
  • Animals, Genetically Modified
  • Herpes Simplex Virus Protein Vmw65
  • Recombinant Fusion Proteins
  • Signal Transduction
  • Xenopus Proteins

MeSH Major

  • Duodenum
  • Liver
  • Pancreas
  • Stomach
  • Transcription Factors
  • Xenopus

abstract

  • Determining the functional attributes of pancreatic transcription factors is essential to understand how the pancreas is specified distinct from other endodermal organs, such as liver, stomach and duodenum, and to direct the differentiation of other cell types into pancreas. Previously, we demonstrated that Pdx1-VP16 was sufficient to convert liver to pancreas. In this paper, we characterize the functional ability of another pancreatic transcription factor, Ptf1a, in promoting ectopic pancreatic fates at early stages throughout the endoderm and later during organogenesis. Using the transthyretin promoter to drive expression in the early liver region/bud of transgenic Xenopus tadpoles, we find that Ptf1a-VP16 is able to convert liver to pancreas. Overexpression of the unmodified Ptf1a on the other hand has no effect in liver but is able to convert stomach and duodenum to pancreas. When overexpressed at earlier embryonic stages throughout the endoderm, Ptf1a activity is similarly limited, whereas Ptf1a-VP16 has increased activity. Interestingly, in all instances we find that Ptf1a-VP16 is only capable of promoting acinar cell fates, whereas Ptf1a promotes both acinar and endocrine fates. Lastly, we demonstrate that, similar to mouse and zebrafish, Xenopus Ptf1a is essential for the initial specification of both endocrine and exocrine cells during normal pancreas development.

publication date

  • April 15, 2007

has subject area

  • Animals
  • Animals, Genetically Modified
  • Duodenum
  • Herpes Simplex Virus Protein Vmw65
  • Liver
  • Pancreas
  • Recombinant Fusion Proteins
  • Signal Transduction
  • Stomach
  • Transcription Factors
  • Xenopus
  • Xenopus Proteins

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4868342

Digital Object Identifier (DOI)

  • 10.1016/j.ydbio.2007.01.027

PubMed ID

  • 17320068

Additional Document Info

start page

  • 786

end page

  • 799

volume

  • 304

number

  • 2