A rapid and sensitive method for determination of sorafenib in human plasma using a liquid chromatography/tandem mass spectrometry assay Academic Article uri icon


MeSH Major

  • Benzenesulfonates
  • Protein Kinase Inhibitors
  • Pyridines
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry


  • A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sorafenib in human plasma. Sample preparation involved a single protein precipitation step by the addition of 0.1 mL of plasma with 0.5 mL acetonitrile. Analysis of the compounds of interest including the internal standard ([(2)H(3)(15)N] sorafenib) was achieved on a Waters X-Terra C(18) (150 mm x 2.1mm i.d., 3.5 microm) analytical column using a mobile phase consisting of acetonitrile/10 mM ammonium acetate (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.2 mL/min for 6 min. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 7.3-7260 ng/mL for the human plasma samples with values for the coefficient of determination of >0.96. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods (<15%).

publication date

  • February 2007



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2006.06.005

PubMed ID

  • 16798122

Additional Document Info

start page

  • 1

end page

  • 7


  • 846


  • 1-2