Low-dose carbon monoxide inhalation prevents ischemia/reperfusion injury of transplanted rat lung grafts. Academic Article uri icon

Overview

MeSH

  • Administration, Inhalation
  • Animals
  • Blood Gas Analysis
  • Drug Administration Schedule
  • Graft Survival
  • Inflammation Mediators
  • Male
  • Rats
  • Rats, Inbred Lew

MeSH Major

  • Carbon Monoxide
  • Lung Transplantation
  • Reperfusion Injury

abstract

  • Carbon monoxide (CO), a byproduct of heme catalysis by heme oxygenases, has been shown to provide protection against ischemia/reperfusion (I/R) injury. We examined the cytoprotective effect of CO at a low concentration on cold I/R injury of transplanted lung grafts. Orthotopic left lung transplantation was performed in syngenic Lewis to Lewis rat combination. Grafts were preserved in University of Wisconsin solution at 4 degrees C for 6 hours. Donors and/or recipients were exposed to CO (250 ppm) in air for 1 hour before surgery and then continuously post-transplantation. Blood oxygen partial pressure of graft pulmonary veins in the CO-treated group versus the air-treated group was significantly higher. The increase of messenger RNA of inflammatory mediators such as interleukin-6, tumor necrosis factor-alpha, inducible nitric oxide synthase, and cycloooxygenase-2 was markedly inhibited in the CO-treated group. The expression of phosphorylated-extracellular signal-regulated protein kinase 1/2 was significantly reduced in the CO-treated group. CO treatment reduced the number of infiltrating macrophages into the lung grafts. Vascular endothelial cells detected by CD31 stain were well preserved in CO-treated grafts, while those in air-treated grafts were faint and interrupted. These results demonstrate that exogenous low-dose CO treatment of donors and recipients can prevent lung I/R injury and significantly improve function of lung grafts after extended cold preservation and transplantation.

publication date

  • August 2006

has subject area

  • Administration, Inhalation
  • Animals
  • Blood Gas Analysis
  • Carbon Monoxide
  • Drug Administration Schedule
  • Graft Survival
  • Inflammation Mediators
  • Lung Transplantation
  • Male
  • Rats
  • Rats, Inbred Lew
  • Reperfusion Injury

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.surg.2006.03.004

PubMed ID

  • 16904967

Additional Document Info

start page

  • 179

end page

  • 185

volume

  • 140

number

  • 2