A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes. Academic Article uri icon

Overview

MeSH

  • Adenoviridae Infections
  • Binding Sites
  • Biological Transport
  • Capsid Proteins
  • Genome, Viral
  • Humans
  • Microtubule-Associated Proteins
  • Parvoviridae Infections
  • Serotyping

MeSH Major

  • Adenoviridae
  • Cytoplasm
  • Dependovirus
  • Dyneins
  • Microtubules

abstract

  • During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.

publication date

  • August 2006

has subject area

  • Adenoviridae
  • Adenoviridae Infections
  • Binding Sites
  • Biological Transport
  • Capsid Proteins
  • Cytoplasm
  • Dependovirus
  • Dyneins
  • Genome, Viral
  • Humans
  • Microtubule-Associated Proteins
  • Microtubules
  • Parvoviridae Infections
  • Serotyping

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1563724

Digital Object Identifier (DOI)

  • 10.1128/JVI.00481-06

PubMed ID

  • 16840360

Additional Document Info

start page

  • 7781

end page

  • 7785

volume

  • 80

number

  • 15