Detection of the JAK2V617F mutation in myeloproliferative disorders by melting curve analysis using the lightcycler system
DNA Mutational Analysis
Molecular Diagnostic Techniques
These findings demonstrate the suitability of this assay for identifying JAK2(V617F) in a clinical laboratory setting. Furthermore, the semiquantitative detection of JAK2(V617F) in archived specimens provides a new tool for studying the prognostic significance of this mutation.