Gene expression profiling of target genes in ventilator-induced lung injury. Academic Article Article uri icon

Overview

MeSH

  • A Kinase Anchor Proteins
  • Amphiregulin
  • Animals
  • Cell Cycle Proteins
  • Cluster Analysis
  • Cysteine-Rich Protein 61
  • DNA-Binding Proteins
  • EGF Family of Proteins
  • Gene Expression Regulation
  • Glycoproteins
  • Immediate-Early Proteins
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-11
  • Lipopolysaccharides
  • Lung
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Reproducibility of Results
  • Transcription Factors

MeSH Major

  • Gene Expression Profiling
  • Lung Injury
  • Respiration, Artificial

abstract

  • In the lungs, high-pressure mechanical ventilation induces an inflammatory response similar to that observed in acute respiratory distress syndrome. To further characterize these responses and to compare them with classical inflammatory pathways, we performed gene expression profiling analysis of 20,000 mouse genes in isolated blood-free (to exclude genes from sequestered leukocytes) perfused mouse lungs exposed to low-pressure ventilation (10 cmH2O), high-pressure ventilation (25 cmH2O, overventilation), and LPS treatment. A large number of inflammatory and apoptotic genes were increased by both overventilation and LPS. However, certain growth factor-related genes, as well as genes related to development, cellular communication, and the cytoskeleton, were only regulated by overventilation. We validated and confirmed increased mRNA expression pattern of five genes (amphiregulin, gravin, Nur77, Cyr61, interleukin-11) by real-time PCR; furthermore, we confirmed increased protein expression of amphiregulin by immunohistochemistry and immunoblotting assays. These genes represent novel candidate genes in ventilator-induced lung injury.

publication date

  • June 16, 2006

has subject area

  • A Kinase Anchor Proteins
  • Amphiregulin
  • Animals
  • Cell Cycle Proteins
  • Cluster Analysis
  • Cysteine-Rich Protein 61
  • DNA-Binding Proteins
  • EGF Family of Proteins
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Glycoproteins
  • Immediate-Early Proteins
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-11
  • Lipopolysaccharides
  • Lung
  • Lung Injury
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Reproducibility of Results
  • Respiration, Artificial
  • Transcription Factors

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1152/physiolgenomics.00110.2005

PubMed ID

  • 16569776

Additional Document Info

start page

  • 68

end page

  • 75

volume

  • 26

number

  • 1