Promoter switching allows simultaneous transcription of LANA and K14/vGPCR of Kaposi's sarcoma-associated herpesvirus. Academic Article uri icon

Overview

MeSH

  • Cell Line
  • Humans
  • Immediate-Early Proteins
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein
  • Mutation
  • Trans-Activators
  • Transcriptional Activation

MeSH Major

  • Antigens, Viral
  • Gene Expression Regulation, Viral
  • Herpesvirus 8, Human
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Viral Proteins

abstract

  • Latent transcription of the latency-associated nuclear antigen (LANA/ORF73) of Kaposi's sarcoma-associated herpesvirus is driven by the LANAp-c. Complexity arises during lytic reactivation, however, as the bicistronic K14/vGPCR transcript initiates 32 bp downstream of LANAp-c in the opposite orientation. We identify an Rta/ORF50-inducible LANA promoter (LANAp-i) that is distinct from the LANAp-c. LANAp-c is unaffected by Rta/ORF50. Utilization of the second, downstream LANAp-i explains how LANA and K14/vGPCR are simultaneously transcribed during de novo infection or lytic reactivation. Transactivation of LANAp-i and K14/vGPCRp requires the C-terminal activation domain of Rta/ORF50 and is mediated by DNA-binding-dependent and -independent Rta/ORF50 mechanisms. Transcriptional profiling following viral reactivation support promoter reporter phenotypes. In sum, cis-elements within the LANAp were selected to ensure faithful expression of LANA and other genes regulated by LANAp during all stages of the KSHV lifecycle despite potential interference from K14/vGPCRp activity.

publication date

  • June 20, 2006

has subject area

  • Antigens, Viral
  • Cell Line
  • Gene Expression Regulation, Viral
  • Herpesvirus 8, Human
  • Humans
  • Immediate-Early Proteins
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein
  • Mutation
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Trans-Activators
  • Transcription, Genetic
  • Transcriptional Activation
  • Viral Proteins

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2006.03.006

PubMed ID

  • 16616289

Additional Document Info

start page

  • 192

end page

  • 205

volume

  • 350

number

  • 1