Epitopes expressed in different adenovirus capsid proteins induce different levels of epitope-specific immunity. Academic Article uri icon

Overview

MeSH

  • Animals
  • Mice
  • Mice, Inbred C57BL
  • Viral Vaccines

MeSH Major

  • Adenoviridae
  • Adenoviridae Infections
  • Capsid Proteins
  • Epitopes
  • Genetic Vectors

abstract

  • On the basis of the concept that the capsid proteins of adenovirus (Ad) gene transfer vectors can be genetically manipulated to enhance the immunogenicity of Ad-based vaccines, the present study compared the antiantigen immunogenicity of Ad vectors with a common epitope of the hemagglutinin (HA) protein of the influenza A virus incorporated into the outer Ad capsid protein hexon, penton base, fiber knob, or protein IX. Incorporation of the same epitope into the different capsid proteins provided insights into the correlation between epitope position and antiepitope immunity. Following immunization of three different strains of mice (C57BL/6, BALB/c, and CBA) with either an equal number of Ad particles (resulting in a different total HA copy number) or different Ad particle numbers (to achieve the same HA copy number), the highest primary (immunoglobulin M [IgM]) and secondary (IgG) anti-HA humoral and cellular CD4 gamma interferon and interleukin-4 responses against HA were always achieved with the Ad vector carrying the HA epitope in fiber knob. These observations suggest that the immune response against an epitope inserted into Ad capsid proteins is not necessarily dependent on the capsid protein number and imply that the choice of incorporation site in Ad capsid proteins in their use as vaccines needs to be compared in vivo.

publication date

  • June 2006

has subject area

  • Adenoviridae
  • Adenoviridae Infections
  • Animals
  • Capsid Proteins
  • Epitopes
  • Genetic Vectors
  • Mice
  • Mice, Inbred C57BL
  • Viral Vaccines

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1472137

Digital Object Identifier (DOI)

  • 10.1128/JVI.02667-05

PubMed ID

  • 16699033

Additional Document Info

start page

  • 5523

end page

  • 5530

volume

  • 80

number

  • 11