Regulation of surface coat exchange by differentiating African trypanosomes. Academic Article uri icon

Overview

MeSH

  • Animals
  • Glycosylphosphatidylinositol Diacylglycerol-Lyase
  • Life Cycle Stages
  • Mice
  • Phosphatidylinositol Diacylglycerol-Lyase

MeSH Major

  • Gene Expression Regulation
  • Membrane Glycoproteins
  • Metalloproteases
  • Protozoan Proteins
  • Trypanosoma brucei brucei
  • Variant Surface Glycoproteins, Trypanosoma

abstract

  • African trypanosomes (Trypanosoma brucei) have a digenetic lifecycle that alternates between the mammalian bloodstream and the tsetse fly vector. In the bloodstream, replicating long slender parasites transform into non-dividing short stumpy forms. Upon transmission into the fly midgut, short stumpy cells differentiate into actively dividing procyclics. A hallmark of this process is the replacement of the bloodstream-stage surface coat composed of variant surface glycoprotein (VSG) with a new coat composed of procyclin. Pre-existing VSG is shed by a zinc metalloprotease activity (MSP-B) and glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC). We now provide a detailed analysis of the coordinate and inverse regulation of these activities during synchronous differentiation. MSP-B mRNA and protein levels are upregulated during differentiation at the same time as proteolysis whereas GPI-PLC levels decrease. When transcription or translation is inhibited, VSG release is incomplete and a substantial amount of protein stays cell-associated. Both modes of release are still evident under these conditions, but GPI hydrolysis plays a quantitatively minor role during normal differentiation. Nevertheless, GPI biosynthesis shifts early in differentiation from a GPI-PLC sensitive structure to a resistant procyclic-type anchor. Translation inhibition also results in a marked increase in the mRNA levels of both MSP-B and GPI-PLC, consistent with negative regulation by labile protein factors. The relegation of short stumpy surface GPI-PLC to a secondary role in differentiation suggests that it may play a more important role as a virulence factor within the mammalian host.

publication date

  • June 2006

has subject area

  • Animals
  • Gene Expression Regulation
  • Glycosylphosphatidylinositol Diacylglycerol-Lyase
  • Life Cycle Stages
  • Membrane Glycoproteins
  • Metalloproteases
  • Mice
  • Phosphatidylinositol Diacylglycerol-Lyase
  • Protozoan Proteins
  • Trypanosoma brucei brucei
  • Variant Surface Glycoproteins, Trypanosoma

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.molbiopara.2006.02.013

PubMed ID

  • 16564583

Additional Document Info

start page

  • 211

end page

  • 223

volume

  • 147

number

  • 2