Viral nanoparticles as tools for intravital vascular imaging Academic Article uri icon

Overview

MeSH Major

  • Comovirus
  • Diagnostic Imaging
  • Endothelium, Vascular
  • Nanostructures

abstract

  • A significant impediment to the widespread use of noninvasive in vivo vascular imaging techniques is the current lack of suitable intravital imaging probes. We describe here a new strategy to use viral nanoparticles as a platform for the multivalent display of fluorescent dyes to image tissues deep inside living organisms. The bioavailable cowpea mosaic virus (CPMV) can be fluorescently labeled to high densities with no measurable quenching, resulting in exceptionally bright particles with in vivo dispersion properties that allow high-resolution intravital imaging of vascular endothelium for periods of at least 72 h. We show that CPMV nanoparticles can be used to visualize the vasculature and blood flow in living mouse and chick embryos to a depth of up to 500 microm. Furthermore, we show that the intravital visualization of human fibrosarcoma-mediated tumor angiogenesis using fluorescent CPMV provides a means to identify arterial and venous vessels and to monitor the neovascularization of the tumor microenvironment.

publication date

  • March 2006

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2536493

Digital Object Identifier (DOI)

  • 10.1038/nm1368

PubMed ID

  • 16501571

Additional Document Info

start page

  • 354

end page

  • 60

volume

  • 12

number

  • 3