Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Electroporation of Competent BAC Host Cells with the Recombinant Shuttle Vector. Academic Article uri icon

Overview

abstract

  • Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

publication date

  • April 1, 2020

Research

keywords

  • Chromosomes, Artificial, Bacterial
  • Electroporation
  • Genetic Engineering
  • Genetic Vectors

Identity

Scopus Document Identifier

  • 85082979042

Digital Object Identifier (DOI)

  • 10.1101/pdb.prot098079

PubMed ID

  • 32238593

Additional Document Info

volume

  • 2020

issue

  • 4