The tumor microenvironment controls primary effusion lymphoma growth in vivo. Academic Article uri icon

Overview

MeSH

  • Abdominal Wall
  • Animals
  • Antiviral Agents
  • Cell Division
  • Exudates and Transudates
  • Ganciclovir
  • Herpesviridae Infections
  • Humans
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation
  • Subrenal Capsule Assay
  • Transcription, Genetic
  • Transplantation, Heterologous

MeSH Major

  • Collagen
  • Drug Combinations
  • Herpesvirus 8, Human
  • Laminin
  • Lymphoma
  • Proteoglycans
  • Xenograft Model Antitumor Assays

abstract

  • Certain lymphomas in AIDS patients, such as primary effusion lymphoma (PEL), are closely associated with the lymphotropic gamma herpes virus Kaposi's sarcoma-associated herpes virus (KSHV), also called human herpesvirus 8. The virus is thought to be essential for tumorigenesis, yet systems to investigate PEL in vivo are rare. Here we describe PEL tumorigenesis in a new xenograft model. Embedded in Matrigel, PEL cells formed rapid, well-organized, and angiogenic tumors after s.c. implantation of C.B.17 SCID mice. Without Matrigel we did not observe comparable tumors, which implies that extracellular support and/or signaling aids PEL. All of the tumors maintained the KSHV genome, and the KSHV latent protein LANA/orf73 was uniformly expressed. However, the expression profile for key lytic mRNAs, as well as LANA-2/vIRF3, differed between tissue culture and sites of implantation. We did not observe a net effect of ganciclovir on PEL growth in culture or as xenograft. These findings underscore the importance of the microenvironment for PEL tumorigenesis and simplify the preclinical evaluation of potential anticancer agents.

publication date

  • July 15, 2004

has subject area

  • Abdominal Wall
  • Animals
  • Antiviral Agents
  • Cell Division
  • Collagen
  • Drug Combinations
  • Exudates and Transudates
  • Ganciclovir
  • Herpesviridae Infections
  • Herpesvirus 8, Human
  • Humans
  • Laminin
  • Lymphoma
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation
  • Proteoglycans
  • Subrenal Capsule Assay
  • Transcription, Genetic
  • Transplantation, Heterologous
  • Xenograft Model Antitumor Assays

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1158/0008-5472.CAN-03-3835

PubMed ID

  • 15256448

Additional Document Info

start page

  • 4790

end page

  • 4799

volume

  • 64

number

  • 14