Inducible nitric oxide synthase mediates prostaglandin h2 synthase nitration and suppresses eicosanoid production. Academic Article uri icon

Overview

MeSH

  • Animals
  • Aorta
  • Apolipoproteins E
  • Blotting, Northern
  • Blotting, Western
  • Cells, Cultured
  • Female
  • Fluorescent Antibody Technique
  • Immunohistochemistry
  • Male
  • Mice
  • Muscle, Smooth, Vascular
  • Myocytes, Smooth Muscle
  • Tyrosine

MeSH Major

  • Coronary Artery Disease
  • Eicosanoids
  • Nitric Oxide Synthase Type II
  • Prostaglandin-Endoperoxide Synthases

abstract

  • Nitric oxide (NO) modulates the biological levels of arachidonate-derived cell signaling molecules by either enhancing or suppressing the activity of prostaglandin H(2) isoforms (PGHS-1 and PGHS-2). Whether NO activates or suppresses PGHS activity is determined by alternative protein modifications mediated by NO and NO-derived species. Here, we show that inducible NO synthase (iNOS) and PGHS-1 co-localize in atherosclerotic lesions of ApoE(-/-) mouse aortae. Immunoblotting and immunohistochemistry revealed Tyr nitration in PGHS-1 in aortic lesions but markedly less in adjacent nonlesion tissue. PGHS-2 was also found in lesions, but 3-nitrotyrosine incorporation was not detected. 3-Nitrotyrosine formation in proteins is considered a hallmark reaction of peroxynitrite, which can form via NO-superoxide reactions in an inflammatory setting. That iNOS-derived NO is essential for 3-nitrotyrosine modification of PGHS-1 was confirmed by the absence of 3-nitrotyrosine in lesions from ApoE(-/-)iNOS(-/-) mice. Mass spectrometric studies specifically identified the active site residue Tyr385 as a 3-nitrotyrosine modification site in purified PGHS-1 exposed to peroxynitrite. PGHS-mediated eicosanoid (PGE(2)) synthesis was more than fivefold accelerated in cultured iNOS(-/-) versus iNOS-expressing mouse aortic smooth muscle cells, suggesting that iNOS-derived NO markedly suppresses PGHS activity in vascular cells. These results further suggest a regulatory role of iNOS in eicosanoid biosynthesis in human atherosclerotic lesions.

publication date

  • January 2006

has subject area

  • Animals
  • Aorta
  • Apolipoproteins E
  • Blotting, Northern
  • Blotting, Western
  • Cells, Cultured
  • Coronary Artery Disease
  • Eicosanoids
  • Female
  • Fluorescent Antibody Technique
  • Immunohistochemistry
  • Male
  • Mice
  • Muscle, Smooth, Vascular
  • Myocytes, Smooth Muscle
  • Nitric Oxide Synthase Type II
  • Prostaglandin-Endoperoxide Synthases
  • Tyrosine

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1592660

Digital Object Identifier (DOI)

  • 10.2353/ajpath.2006.050090

PubMed ID

  • 16400036

Additional Document Info

start page

  • 349

end page

  • 362

volume

  • 168

number

  • 1