Volumetric tomography of fluorescent proteins through small animals in vivo Academic Article uri icon

Overview

MeSH Major

  • Luminescent Proteins
  • Tomography

abstract

  • Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We demonstrate noninvasive imaging of lung tumor progression in a murine model, as well as imaging of gene delivery using a herpes virus vector. This technology can significantly improve imaging capacity over the current state of the art and should find wide in vivo imaging applications in drug discovery, immunology, and cancer research.

publication date

  • December 20, 2005

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1317905

Digital Object Identifier (DOI)

  • 10.1073/pnas.0504628102

PubMed ID

  • 16344470

Additional Document Info

start page

  • 18252

end page

  • 7

volume

  • 102

number

  • 51