Validation of Anti-Mouse PDL-1 Goat Polyclonal Antibody Staining with Mouse PDL-1 In Situ Hybridization on Adjacent Sections of Cell Pellets and Mouse Tumors. Article uri icon

Overview

abstract

  • Finding a valid antibody to detect mouse programmed death ligand 1 (PDL-1) by immunohistochemistry or immunofluorescence staining has been notoriously difficult. Successful validation of an antibody requires the use of multiple detection methods with the ability to compare appropriate positive and negative controls. Here, we describe in detail the protocols used to validate a mouse-specific PDL-1 antibody used in immunohistochemistry staining with an mRNA in situ hybridization on adjacent sections of mouse B16 tumor. This validation is supported by immunohistochemistry staining of PDL-1 on B16 cell pellets either treated or not treated with IFN-gamma.

publication date

  • January 1, 2017

Research

keywords

  • Antibodies
  • B7-H1 Antigen
  • Biomarkers, Tumor
  • In Situ Hybridization
  • Neoplasms

Identity

PubMed Central ID

  • PMC5751444

Scopus Document Identifier

  • 85012986105

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-6759-9_18

PubMed ID

  • 28185198

Additional Document Info

volume

  • 1554