Human endogenous antibiotic LL-37 stimulates airway epithelial cell proliferation and wound closure. Academic Article uri icon

Overview

MeSH

  • Base Sequence
  • Cell Line
  • Cell Proliferation
  • DNA, Complementary
  • Epithelial Cells
  • Gene Expression
  • Humans
  • Receptors, Formyl Peptide
  • Receptors, Leukotriene B4
  • Receptors, Lipoxin
  • Receptors, Purinergic P2
  • Signal Transduction
  • Wound Healing

MeSH Major

  • Antimicrobial Cationic Peptides
  • Bronchi

abstract

  • Antimicrobial peptides are endogenous antibiotics that directly inactivate microorganisms and in addition have a variety of receptor-mediated functions. LL-37/hCAP-18 is the only cathelicidin found in humans and is involved in angiogenesis and regulation of the innate immune system. The aim of the present study was to characterize the role of the peptide LL-37 in the regulation of wound closure of the airway epithelium in the cell line NCI-H292 and primary airway epithelial cells. LL-37 stimulated healing of mechanically induced wounds in monolayers of the cell line and in differentiated primary airway epithelium. This effect was detectable at concentrations of 5 mug/ml in NCI-H292 and 1 mug/ml in primary cells. The effect of LL-37 on wound healing was dependent on the presence of serum. LL-37 induced cell proliferation and migration of NCI-H292 cells. Inhibitor studies in the wound closure and proliferation assays indicated that the effects caused by LL-37 are mediated through epidermal growth factor receptor, a G protein-coupled receptor, and MAP/extracellular regulated kinase. In conclusion, LL-37 induces wound healing, proliferation, and migration of airway epithelial cells. The peptide is likely involved in the regulation of tissue homeostasis in the airways.

publication date

  • November 2005

has subject area

  • Antimicrobial Cationic Peptides
  • Base Sequence
  • Bronchi
  • Cell Line
  • Cell Proliferation
  • DNA, Complementary
  • Epithelial Cells
  • Gene Expression
  • Humans
  • Receptors, Formyl Peptide
  • Receptors, Leukotriene B4
  • Receptors, Lipoxin
  • Receptors, Purinergic P2
  • Signal Transduction
  • Wound Healing

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1152/ajplung.00286.2004

PubMed ID

  • 15964896

Additional Document Info

start page

  • L842

end page

  • L848

volume

  • 289

number

  • 5