Intrapleural 'outside-in' gene therapy: therapeutics for organs of the chest via gene transfer to the pleura. Review uri icon

Overview

MeSH

  • Adenoviridae
  • Dependovirus
  • Gene Transfer Techniques
  • Genetic Vectors

MeSH Major

  • Genetic Therapy
  • Immunologic Factors
  • Lung Neoplasms
  • Mesothelioma
  • Pleura
  • alpha 1-Antitrypsin
  • alpha 1-Antitrypsin Deficiency

abstract

  • The pleural space is an attractive site for using viral vectors to deliver gene products to the lung parenchyma, other thoracic structures and the systemic circulation. The advantages of intrapleural gene transfer using viral vectors include: (i) easy accessibility; (ii) large surface area; (iii) ability to provide high concentrations of secreted gene products to chest structures; (iv) low risk of detrimental effects of possible vector-induced inflammation compared with intravascular delivery; and (v) because it is local, lower vector doses can be used to deliver therapeutic genes to thoracic structures than less efficient systemic routes. Examples of pleural gene transfer include the use of adenovirus vectors to treat mesothelioma by transiently expressing genes that encode toxic proteins, immunomodulatory molecules or anti-angiogenesis factors. Intrapleural delivery of adeno-associated viral vectors represents an efficient strategy to treat alpha1-antitrypsin (alpha1AT) deficiency, achieving high lung and systemic therapeutic levels of alpha1AT. Intrapleural delivery of gene transfer vectors holds promise for the treatment of diseases requiring transient, localized gene expression, as well as sustained expression of genes to correct hereditary disorders requiring localized or systemic expression of the therapeutic protein.

publication date

  • October 2005

has subject area

  • Adenoviridae
  • Dependovirus
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Vectors
  • Immunologic Factors
  • Lung Neoplasms
  • Mesothelioma
  • Pleura
  • alpha 1-Antitrypsin
  • alpha 1-Antitrypsin Deficiency

Research

keywords

  • Journal Article
  • Review

Identity

Language

  • eng

PubMed ID

  • 16248279

Additional Document Info

start page

  • 440

end page

  • 453

volume

  • 7

number

  • 5