Use of gene expression profiling to direct in vivo molecular imaging of lung cancer Academic Article uri icon


MeSH Major

  • Adenocarcinoma, Bronchiolo-Alveolar
  • Cathepsins
  • Diagnostic Imaging
  • Gene Expression Profiling
  • Lung Neoplasms
  • Up-Regulation


  • Using gene expression profiling, we identified cathepsin cysteine proteases as highly up-regulated genes in a mouse model of human lung adenocarcinoma. Overexpression of cathepsin proteases in these lung tumors was confirmed by immunohistochemistry and Western blotting. Therefore, an optical probe activated by cathepsin proteases was selected to detect murine lung tumors in vivo as small as 1 mm in diameter and spatially separated. We generated 3D maps of the fluorescence signal and fused them with anatomical computed tomography images to show a close correlation between fluorescence signal and tumor burden. By serially imaging the same mouse, optical imaging was used to follow tumor progression. This study demonstrates the capability for molecular imaging of a primary lung tumor by using endogenous proteases expressed by a tumor. It also highlights the feasibility of using gene expression profiling to identify molecular targets for imaging lung cancer.

publication date

  • October 4, 2005



  • Academic Article



  • eng

PubMed Central ID

  • PMC1242291

Digital Object Identifier (DOI)

  • 10.1073/pnas.0503920102

PubMed ID

  • 16183744

Additional Document Info

start page

  • 14404

end page

  • 9


  • 102


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