Coenzyme Q10 and lipid-related gene induction in HeLa cells. Academic Article uri icon

Overview

MeSH

  • Coenzymes
  • Female
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells
  • Humans
  • Lipid Peroxidation
  • Oxidation-Reduction
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Reference Values
  • Sensitivity and Specificity
  • Transcriptional Activation
  • Uterine Cervical Neoplasms

MeSH Major

  • Antioxidants
  • Apoptosis
  • Cell Division
  • Ubiquinone

abstract

  • Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. We investigated the cellular and molecular effects of 30 micromol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

publication date

  • May 2004

has subject area

  • Antioxidants
  • Apoptosis
  • Cell Division
  • Coenzymes
  • Female
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells
  • Humans
  • Lipid Peroxidation
  • Oxidation-Reduction
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Reference Values
  • Sensitivity and Specificity
  • Transcriptional Activation
  • Ubiquinone
  • Uterine Cervical Neoplasms

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.ajog.2004.01.076

PubMed ID

  • 15167860

Additional Document Info

start page

  • 1432

end page

  • 1434

volume

  • 190

number

  • 5