Self-assembly of carbon nanotubes and antibodies on tumours for targeted amplified delivery. Academic Article uri icon

Overview

abstract

  • Single-walled carbon nanotubes (SWNTs) can deliver imaging agents or drugs to tumours and offer significant advantages over approaches based on antibodies or other nanomaterials. In particular, the nanotubes can carry a substantial amount of cargo (100 times more than a monoclonal antibody), but can still be rapidly eliminated from the circulation by renal filtration, like a small molecule, due to their high aspect ratio. Here we show that SWNTs can target tumours in a two-step approach in which nanotubes modified with morpholino oligonucleotide sequences bind to cancer cells that have been pretargeted with antibodies modified with oligonucleotide strands complementary to those on the nanotubes. The nanotubes can carry fluorophores or radioisotopes, and are shown to selectively bind to cancer cells in vitro and in tumour-bearing xenografted mice. The binding process is also found to lead to antigen capping and internalization of the antibody-nanotube complexes. The nanotube conjugates were labelled with both alpha-particle and gamma-ray emitting isotopes, at high specific activities. Conjugates labelled with alpha-particle-generating (225)Ac were found to clear rapidly, thus mitigating radioisotope toxicity, and were shown to be therapeutically effective in vivo.

publication date

  • September 29, 2013

Research

keywords

  • Antibodies, Monoclonal
  • Drug Delivery Systems
  • Nanotubes, Carbon

Identity

PubMed Central ID

  • PMC3798027

Scopus Document Identifier

  • 84885481781

Digital Object Identifier (DOI)

  • 10.1038/nnano.2013.190

PubMed ID

  • 24077028

Additional Document Info

volume

  • 8

issue

  • 10