Caveolin-1 expression by means of p38beta mitogen-activated protein kinase mediates the antiproliferative effect of carbon monoxide. Academic Article Article uri icon

Overview

MeSH

  • Animals
  • Blotting, Western
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Cyclin-Dependent Kinase Inhibitor p21
  • Enzyme Activation
  • Fibroblasts
  • Gene Transfer Techniques
  • Guanylate Cyclase
  • Heme Oxygenase (Decyclizing)
  • Immunohistochemistry
  • Immunoprecipitation
  • RNA, Small Interfering
  • Rats
  • Rats, Inbred Lew
  • Thymidine
  • Tritium

MeSH Major

  • Aorta
  • Carbon Monoxide
  • Gene Expression Regulation
  • Mitogen-Activated Protein Kinase 11
  • Muscle, Smooth
  • Signal Transduction

abstract

  • During vascular injury, the proliferation and migration of smooth muscle cells leads to characteristic neointima formation, which can be exacerbated by genetic depletion of caveolin-1 or heme oxygenase 1 (HO-1), and inhibited by carbon monoxide (CO), a by-product of heme oxygenase 1 activity. CO inhibited smooth muscle cell proliferation by activating p38 mitogen-activated protein kinase (MAPK) and p21(Waf1/Cip1). Exposure to CO increased caveolin-1 expression in neointimal lesions of injured aorta and in vitro by activating guanylyl cyclase and p38 MAPK. p38beta-/- fibroblasts did not induce caveolin-1 in response to CO, and exhibited a diminished basal caveolin-1 expression, which was restored by p38beta gene transfer. p38beta MAPK down-regulated extracellular signal-regulated protein kinase 1/2 (ERK-1/2), which can repress caveolin-1 transcription. Genetic depletion of caveolin-1 abolished the antiproliferative effect of CO. Thus, we demonstrate that CO, by activating p38beta MAPK, up-regulates caveolin-1, which acts as a tumor suppressor protein that mediates the growth inhibitory properties of this gas.

publication date

  • August 9, 2005

has subject area

  • Animals
  • Aorta
  • Blotting, Western
  • Carbon Monoxide
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Cyclin-Dependent Kinase Inhibitor p21
  • Enzyme Activation
  • Fibroblasts
  • Gene Expression Regulation
  • Gene Transfer Techniques
  • Guanylate Cyclase
  • Heme Oxygenase (Decyclizing)
  • Immunohistochemistry
  • Immunoprecipitation
  • Mitogen-Activated Protein Kinase 11
  • Muscle, Smooth
  • RNA, Small Interfering
  • Rats
  • Rats, Inbred Lew
  • Signal Transduction
  • Thymidine
  • Tritium

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1183544

Digital Object Identifier (DOI)

  • 10.1073/pnas.0501345102

PubMed ID

  • 16051704

Additional Document Info

start page

  • 11319

end page

  • 11324

volume

  • 102

number

  • 32