Neuronally expressed stem cell factor induces neural stem cell migration to areas of brain injury Academic Article Article uri icon

Overview

MeSH Major

  • Glioblastoma
  • Surveys and Questionnaires

abstract

  • Neural stem/progenitor cell (NSPC) migration toward sites of damaged central nervous system (CNS) tissue may represent an adaptive response for the purpose of limiting and/or repairing damage. Little is known of the mechanisms responsible for this migratory response. We constructed a cDNA library of injured mouse forebrain using subtractive suppression hybridization (SSH) to identify genes that were selectively upregulated in the injured hemisphere. We demonstrate that stem cell factor (SCF) mRNA and protein are highly induced in neurons within the zone of injured brain. Additionally, the SCF receptor c-kit is expressed on NSPCs in vitro and in vivo. Finally, we demonstrate that recombinant SCF induces potent NSPC migration in vitro and in vivo through the activation of c-kit on NSPCs. These data suggest that the SCF/c-kit pathway is involved in the migration of NSPCs to sites of brain injury and that SCF may prove useful for inducing progenitor cell recruitment to specific areas of the CNS for cell-based therapeutic strategies.

publication date

  • May 2004

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1172/JCI200420001

PubMed ID

  • 15124028

Additional Document Info

start page

  • 1364

end page

  • 74

volume

  • 113

number

  • 9