Similarity of gene expression patterns in human alveolar macrophages in response to Pseudomonas aeruginosa and Burkholderia cepacia. Academic Article uri icon

Overview

MeSH

  • Burkholderia cepacia
  • Down-Regulation
  • Gene Expression Profiling
  • Humans
  • Pseudomonas aeruginosa
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation

MeSH Major

  • Burkholderia Infections
  • Gene Expression
  • Macrophages, Alveolar
  • Pseudomonas Infections

abstract

  • To determine if differences in the severity of pulmonary infection in cystic fibrosis seen with late isolates of Pseudomonas aeruginosa and Burkholderia cepacia are associated with differences in the initial response of alveolar macrophages (AM) to these pathogens, we assessed gene expression changes in human AM in response to infection with a laboratory strain, early and late clinical isolates of P. aeruginosa, and B. cepacia. Analysis of gene expression changes at the RNA level using oligonucleotide microarrays, following exposure to laboratory P. aeruginosa strain PAK, showed significant (P < 0.01) >2.5-fold upregulation of 42 genes and >2.5-fold downregulation of 45 genes. The majority of the changes in gene expression involved genes as part of inflammatory pathways and signaling systems. Interestingly, similar responses were observed following exposure of AM to early and late clinical isolates of P. aeruginosa, as well as with B. cepacia, suggesting that the more severe clinical outcome of infections with late clinical isolates of P. aeruginosa or with B. cepacia cannot be explained by differences in the early interactions of these organisms with the human AM, as reflected by the similarity of gene expression changes in response to exposure of AM to these pathogens.

publication date

  • August 2005

has subject area

  • Burkholderia Infections
  • Burkholderia cepacia
  • Down-Regulation
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Macrophages, Alveolar
  • Pseudomonas Infections
  • Pseudomonas aeruginosa
  • Reverse Transcriptase Polymerase Chain Reaction
  • Up-Regulation

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1201277

Digital Object Identifier (DOI)

  • 10.1128/IAI.73.8.5262-5268.2005

PubMed ID

  • 16041053

Additional Document Info

start page

  • 5262

end page

  • 5268

volume

  • 73

number

  • 8