Alterations in nitric oxide and cytokine production with airway inflammation in the absence of IL-10. Academic Article uri icon

Overview

MeSH

  • Animals
  • Bronchial Hyperreactivity
  • Bronchoalveolar Lavage Fluid
  • Cell Movement
  • Cells, Cultured
  • Exhalation
  • Interleukin-18
  • Interleukin-4
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nitrites

MeSH Major

  • Cytokines
  • Interleukin-10
  • Nitric Oxide
  • Respiratory Hypersensitivity

abstract

  • IL-10 is an anti-inflammatory cytokine that suppresses NO synthase (NOS) and production of NO; its lack may promote NO production and alterations in cytokines modulated by NO with allergic airway inflammation (AI), such as IL-18 and IL-4. Therefore, we induced AI in IL-10 knockout ((-/-)) and IL-10-sufficient C57BL/6 (C57) mice with inhaled OVA and measured airway NO production, as exhaled NO (E(NO)) and bronchoalveolar lavage fluid nitrite levels. E(NO) and nitrite levels were elevated significantly in naive IL-10(-/-) mice as compared with C57 mice. With AI, E(NO) and nitrite levels increased in C57 mice and decreased in IL-10(-/-) mice. IL-18 production fell with both AI and addition of S-nitroso-N-acetyl-d,l-penicillamine (a NO donor) but was not significantly increased by chemical NOS inhibition by l-N(5)-(1-iminoethyl)-ornithine. IL-4 AI was increased significantly (up to 10-fold greater) in the absence of IL-10 but was reduced significantly with chemical inhibition of NOS. Airway responsiveness was lower in IL-10(-/-) mice and was associated with alteration in production of NO and IL-4. Thus, IL-4 production was increased, and likely decreased NO production, in a way not predicted by the absence of IL-10. Inhibition of IL-4 production, with inhibition of NOS in the absence of IL-10, demonstrated the importance of a NO and IL-4 feedback mechanism regulating this interaction.

publication date

  • July 15, 2005

has subject area

  • Animals
  • Bronchial Hyperreactivity
  • Bronchoalveolar Lavage Fluid
  • Cell Movement
  • Cells, Cultured
  • Cytokines
  • Exhalation
  • Interleukin-10
  • Interleukin-18
  • Interleukin-4
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Nitric Oxide
  • Nitrites
  • Respiratory Hypersensitivity

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 16002724

Additional Document Info

start page

  • 1206

end page

  • 1213

volume

  • 175

number

  • 2