Dosage-dependent requirement for mouse Vezf1 in vascular system development. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • Embryonic Development
  • Endothelium, Vascular
  • Gene Dosage
  • Genotype
  • Kruppel-Like Transcription Factors
  • Mesoderm
  • Mice
  • Mice, Knockout
  • Neovascularization, Physiologic
  • Polymerase Chain Reaction
  • Yolk Sac

MeSH Major

  • Blood Vessels
  • Gene Expression Regulation, Developmental
  • Transcription Factors

abstract

  • Vezf1 is an early development gene that encodes a zinc finger transcription factor. In the developing embryo, Vezf1 is expressed in the yolk sac mesoderm and the endothelium of the developing vasculature and, in addition, in mesodermal and neuronal tissues. Targeted inactivation of Vezf1 in mice reveals that it acts in a closely regulated, dose-dependent fashion on the development of the blood vascular and lymphatic system. Homozygous mutant embryos display vascular remodeling defects and loss of vascular integrity leading to localized hemorrhaging. Ultrastructural analysis shows defective endothelial cell adhesion and tight junction formation in the mutant vessels. Moreover, in heterozygous embryos, haploinsufficiency is observed that is characterized by lymphatic hypervascularization associated with hemorrhaging and edema in the jugular region; a phenotype reminiscent of the human congenital lymphatic malformation syndrome cystic hygroma.

publication date

  • July 1, 2005

has subject area

  • Animals
  • Base Sequence
  • Blood Vessels
  • Cloning, Molecular
  • DNA Primers
  • Embryonic Development
  • Endothelium, Vascular
  • Gene Dosage
  • Gene Expression Regulation, Developmental
  • Genotype
  • Kruppel-Like Transcription Factors
  • Mesoderm
  • Mice
  • Mice, Knockout
  • Neovascularization, Physiologic
  • Polymerase Chain Reaction
  • Transcription Factors
  • Yolk Sac

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1453095

Digital Object Identifier (DOI)

  • 10.1016/j.ydbio.2005.04.003

PubMed ID

  • 15882861

Additional Document Info

start page

  • 140

end page

  • 156

volume

  • 283

number

  • 1