Determination of SU5416, a novel angiogenesis inhibitor, in human plasma by liquid chromatography Academic Article uri icon

Overview

MeSH Major

  • Angiogenesis Inhibitors
  • Chromatography, High Pressure Liquid
  • Indoles
  • Pyrroles
  • Spectrophotometry, Ultraviolet

abstract

  • A high-performance liquid chromatographic (HPLC) assay with UV detection has been developed for the quantitative determination of the antiangiogenic agent SU5416 in human plasma. Sample pretreatment involved a single protein-precipitation step with acetonitrile containing the internal standard, chrysin. Separation of the compounds of interest was achieved on a column packed with HP Zorbax C(8) material (5microm particle size; length: 150mm; i.d.: 4.6mm) using a dual solvent system of 0.01M aqueous ammonium acetate and acetonitrile delivered as a nonlinear gradient at a flow-rate of 1.00ml/min. Simultaneous UV detection was performed at 440nm (SU5416) and 268nm (chrysin). The calibration graph was fit to log-transformed response-concentration data over a range of 10-5000ng/ml. Values for accuracy and precision, obtained from six quality controls analyzed on different days in replicates of 3 or 6, ranged 92.9-109 and 0.8-6.2%, respectively. The developed method was successfully applied to study the pharmacokinetics of SU5416 in a cancer patient receiving the drug as a 1h infusion.

publication date

  • June 5, 2004

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.jchromb.2004.02.036

PubMed ID

  • 15113549

Additional Document Info

start page

  • 135

end page

  • 40

volume

  • 805

number

  • 1