Identification of murine CD8 T cell epitopes in codon-optimized SARS-associated coronavirus spike protein. Academic Article uri icon

Overview

MeSH

  • Adenoviruses, Human
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cercopithecus aethiops
  • Codon
  • Genetic Vectors
  • H-2 Antigens
  • Histocompatibility Antigen H-2D
  • Humans
  • Immunization
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Peptides
  • Severe Acute Respiratory Syndrome
  • Spike Glycoprotein, Coronavirus
  • Vero Cells
  • Viral Vaccines

MeSH Major

  • CD8-Positive T-Lymphocytes
  • Epitope Mapping
  • Epitopes, T-Lymphocyte
  • Membrane Glycoproteins
  • SARS Virus
  • Viral Envelope Proteins

abstract

  • The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus, SARS-associated coronavirus (SARS-CoV). CD8 T cells play an important role in controlling diseases caused by other coronaviruses and in mediating vaccine-induced protective immunity in corresponding animal models. The spike protein, a main surface antigen of SARS-CoV, is one of the most important antigen candidates for vaccine design. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mice immunized with vectors encoding codon-optimized SARS-CoV spike protein. CD8 T-cell responses were mapped to two H-2(b)-restricted epitopes (S436-443 and S525-532) and one H-2(d)-restricted epitope (S366-374). The identification of these epitopes will facilitate the evaluation of vaccine strategies in murine models of SARS-CoV infection. Furthermore, codon and promoter optimizations can greatly enhance the overall immunogenicity of spike protein in the context of replication-defective human and simian adenoviral vaccine carriers. The optimized recombinant adenoviral vaccine vectors encoding spike can generate robust antigen-specific cellular immunity in mice and may potentially be useful for control of SARS-CoV infection.

publication date

  • April 25, 2005

has subject area

  • Adenoviruses, Human
  • Amino Acid Sequence
  • Animals
  • CD8-Positive T-Lymphocytes
  • Cell Line
  • Cercopithecus aethiops
  • Codon
  • Epitope Mapping
  • Epitopes, T-Lymphocyte
  • Genetic Vectors
  • H-2 Antigens
  • Histocompatibility Antigen H-2D
  • Humans
  • Immunization
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Peptides
  • SARS Virus
  • Severe Acute Respiratory Syndrome
  • Spike Glycoprotein, Coronavirus
  • Vero Cells
  • Viral Envelope Proteins
  • Viral Vaccines

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2005.01.050

PubMed ID

  • 15823604

Additional Document Info

start page

  • 34

end page

  • 45

volume

  • 335

number

  • 1