Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia Academic Article uri icon

Overview

MeSH Major

  • Chromosomes, Human, Pair 13
  • Gene Deletion
  • Gene Expression Regulation, Leukemic
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • MicroRNAs
  • RNA, Neoplasm

abstract

  • Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.

publication date

  • November 26, 2002

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC137750

Digital Object Identifier (DOI)

  • 10.1073/pnas.242606799

PubMed ID

  • 12434020

Additional Document Info

start page

  • 15524

end page

  • 9

volume

  • 99

number

  • 24