Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cells, Cultured
  • Chemokine CXCL12
  • Chemokines
  • Chemokines, CXC
  • Endothelial Growth Factors
  • Female
  • Fluorouracil
  • Immunosuppressive Agents
  • Lymphokines
  • Male
  • Megakaryocytes
  • Mice
  • Mice, Knockout
  • Mice, SCID
  • Myeloid Cells
  • Recovery of Function
  • Stem Cells
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

MeSH Major

  • Bone Marrow
  • Cell Differentiation
  • Cell Movement
  • Hematopoietic Stem Cells
  • Matrix Metalloproteinase 9
  • Stem Cell Factor

abstract

  • Stem cells within the bone marrow (BM) exist in a quiescent state or are instructed to differentiate and mobilize to circulation following specific signals. Matrix metalloproteinase-9 (MMP-9), induced in BM cells, releases soluble Kit-ligand (sKitL), permitting the transfer of endothelial and hematopoietic stem cells (HSCs) from the quiescent to proliferative niche. BM ablation induces SDF-1, which upregulates MMP-9 expression, and causes shedding of sKitL and recruitment of c-Kit+ stem/progenitors. In MMP-9-/- mice, release of sKitL and HSC motility are impaired, resulting in failure of hematopoietic recovery and increased mortality, while exogenous sKitL restores hematopoiesis and survival after BM ablation. Release of sKitL by MMP-9 enables BM repopulating cells to translocate to a permissive vascular niche favoring differentiation and reconstitution of the stem/progenitor cell pool.

publication date

  • May 31, 2002

has subject area

  • Animals
  • Bone Marrow
  • Cell Differentiation
  • Cell Movement
  • Cells, Cultured
  • Chemokine CXCL12
  • Chemokines
  • Chemokines, CXC
  • Endothelial Growth Factors
  • Female
  • Fluorouracil
  • Hematopoietic Stem Cells
  • Immunosuppressive Agents
  • Lymphokines
  • Male
  • Matrix Metalloproteinase 9
  • Megakaryocytes
  • Mice
  • Mice, Knockout
  • Mice, SCID
  • Myeloid Cells
  • Recovery of Function
  • Stem Cell Factor
  • Stem Cells
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC2826110

PubMed ID

  • 12062105

Additional Document Info

start page

  • 625

end page

  • 637

volume

  • 109

number

  • 5