Amiloride-sensitive Na channels from the apical membrane of the rat cortical collecting tubule Academic Article uri icon

Overview

MeSH Major

  • Ion Channels
  • Kidney Tubules
  • Kidney Tubules, Collecting
  • Sodium

abstract

  • Currents through individual Na channels in the apical membrane of the rat cortical collecting tubule were resolved by using the patch-clamp technique. In cell-attached patches, the channels had a conductance of 5 pS with 140 mM NaCl in the pipet. The conductance was a saturable function of external Na, with a maximal value of about 8 pS and a half saturation at about 75 mM Na. In excised inside-out patches, the selectivity of the channels for Na over K was estimated from reversal potentials to be at least 10:1. The channels underwent spontaneous transitions between open and closed states. Both states had mean lifetimes of 3-4 sec. Amiloride (0.5 microM) added to the pipet induced more frequent closures and openings of the channels and a reduction in the mean open time. These channels are presumed to mediate Na reabsorption by this nephron segment in vivo.

publication date

  • January 1986

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC323382

PubMed ID

  • 2422661

Additional Document Info

start page

  • 2767

end page

  • 70

volume

  • 83

number

  • 8